FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16476072
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGGGGGT269910.16381938607697272TruSeq Adapter, Index 21 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG172500.10469728464405836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG171100.055.05118628
GTCACGT176200.053.04059229
CGTTTCG189000.048.74362633
CAGTCAC195500.048.28528227
CGGAATC186700.047.5609138
GTTTCGG194000.047.4147534
CCAGTCA200550.047.06899326
GCGGGGT44400.046.1967152
TCCAGTC205850.045.85625
ACTCCAG207350.045.53866223
TCGGGGG51750.044.84282743
GAACTCC213400.044.47600621
ACGTCTG215600.044.3792815
ATCGGGG60150.044.28318442
CGTCTGA217200.044.10056316
TCACGTT212800.044.03274530
CGGGGGT45450.043.81944744
ACGTTTC214100.043.29100832
ACACGTC224750.042.75937713
CACGTCT225000.042.54063814
Produced by FastQC (version 0.11.5)