FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15285451
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT3008141.9679759530811358TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA383450.066.6878936
GCCTTAT393850.064.71364637
CGTGGCC401650.063.9729433
CGTATGC332200.063.92242446
AGTCACG413500.063.64570628
GTATGCC338450.063.45473547
ATGCCGT320550.063.3730849
TGGCCTT404400.063.26835335
TATGCCG334250.063.1425248
GTCACGT415950.063.1027429
CTCGTAT349300.062.86750844
GTGGCCT407250.062.843634
TGCCGTC319000.062.78151350
TCGTATG348700.062.37342545
GCCGTCT300200.061.86444551
CCTTATC412600.061.47592538
CAGTCAC432800.061.1373127
CTTATCT388850.060.5411739
TCACGTG434350.060.22811530
CCAGTCA440600.060.0937226
Produced by FastQC (version 0.11.5)