FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18899075
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT10855245.743794339140937TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1267600.067.2136346
TATGCCG1269850.067.0662648
GTATGCC1274150.066.97603647
AGTCACG1288250.066.8610628
ATGCCGT1274200.066.680249
GTCACGT1287500.066.669129
CTCGTAT1262350.066.6057244
TCGTATG1275850.066.523745
CGATCTC1216500.066.3495740
GATCTCG1241950.066.1724541
ACGATCT1221550.066.0182139
TGCCGTC1283650.065.960150
AACGATC1229500.065.8845638
CAGTCAC1308800.065.79613527
ACACGTC1321750.065.779913
CCAGTCA1306250.065.682926
ACGTCTG1321050.065.6632215
TCACGTG1305350.065.6314130
CACGTGA1303200.065.6124131
ACTCCAG1303000.065.4910323
Produced by FastQC (version 0.11.5)