FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17557951
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT10325065.880560892327356TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG1267750.066.79761532
CGTCCGC1262550.066.6317333
CGTATGC1222850.066.60750646
GTATGCC1230850.066.539947
GTCCGCA1258900.066.5109234
TCCGCAC1252000.066.4871635
TATGCCG1227100.066.4087648
CACGTCC1279750.066.3760231
AGTCACG1306700.066.3232428
ATGCCGT1216500.066.1670349
GTCACGT1308400.066.1061629
TCGTATG1237100.066.0130445
CTCGTAT1237250.066.00239644
CGCACAT1246100.065.83362637
TCACGTC1306800.065.7365530
TGCCGTC1213800.065.5556350
ACACGTC1328250.065.5411213
CAGTCAC1322000.065.4997227
CCAGTCA1315800.065.41447426
TCCAGTC1317350.065.3874225
Produced by FastQC (version 0.11.5)