FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16806469
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT13450718.003293255710048TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTAT311440.18530959715571427TruSeq Adapter, Index 16 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTAT185910.1106181197252082TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG1545600.068.0848735
GTCCCGA1511150.067.9013636
CCGTCCC1553500.067.8422534
TCACCCG1571350.067.6626930
ACCCGTC1564600.067.65320632
CCCGATC1477200.067.643438
GTCACCC1574400.067.6316529
CACCCGT1570000.067.5502231
CGTATGC1556050.067.4894146
TATGCCG1554150.067.4818448
GTATGCC1561050.067.4130947
AGTCACC1594950.067.3420328
ATGCCGT1547750.067.2968949
ACGTCTG1622300.067.2872815
TCGTATG1560650.067.133345
CTCGTAT1544900.067.1287844
ACACGTC1631550.067.0696213
CGTCTGA1627350.067.05421416
CACGTCT1630150.067.0304114
GCACACG1634850.067.00059511
Produced by FastQC (version 0.11.5)