FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18222304
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT12524376.873099032921413TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT915190.5022361606962544TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTGT504020.2765951001585749TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGTAT381960.2096112544275411TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGGAT313860.1722394709253012TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTTT311220.17079069693931132TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTGT293570.1611047647981287TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGTGT220920.12123604128215619TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGGAT207990.11414034141895557TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGGGT202580.11117145230372626TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG1985650.067.355115
ACACGTC1993000.067.2841513
GCACACG1995600.067.249111
CACACGT1998600.067.1446512
CACGTCT1993500.067.1297814
CGTCTGA1993950.067.0380616
CCGTATC1909350.066.8944638
CGTATGC1305300.066.8396546
CGTATCT1631550.066.8309339
TATGCCG1310350.066.78148
GTCACAG1968900.066.7743729
ACTCCAG1979850.066.7569323
GTTCCGT1935950.066.72526635
CTCGTAT1387950.066.68008444
GTATGCC1341800.066.666547
CAGTCAC1986600.066.66012627
TCCGTAT1929200.066.64159437
TCCAGTC1983100.066.61764525
AGTTCCG1941850.066.6121534
GAACTCC1994050.066.60876521
Produced by FastQC (version 0.11.5)