FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16432844
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT6097713.710684529105248TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC686700.065.48746546
ATGCCGT676650.065.35831549
GTATGCC691100.065.2664647
TATGCCG687350.065.2063648
TGCCGTC673900.064.9184650
GCCGTCT642700.064.9065851
CTCGTAT698900.064.585344
TCGTATG704600.064.38035645
CACAGTC736200.064.1193231
GTCACAG742400.063.8963829
CAGTCAC753900.063.336127
CCAGTCA753150.063.24619726
AGTCAAC742050.062.9866634
CCGTCTT665400.062.88167652
AGTCACA761750.062.62251328
ACTCCAG762100.062.58040223
ACAGTCA752900.062.5706232
TCTCGTA722000.062.5640843
TCCAGTC761850.062.50481825
GTCAACA753850.061.8114335
Produced by FastQC (version 0.11.5)