FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17229225
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2883701.6737258930683185TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC302040.17530678251633489TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC377950.064.67667444
GTATGCC383500.064.0325545
TATGCCG377400.063.93625346
AGTCACC505150.063.41652328
ATGCCGT345550.063.12269647
GTCACCT506850.062.9684729
CCTTGTA504250.062.94497733
TCGTATG399050.062.8447843
CTCGTAT366350.062.40729542
CACCTTG513000.062.0424731
ACCTTGT512350.061.9838232
TGCCGTC312300.061.76429448
CAGTCAC527400.061.02401427
GCCGTCT298700.061.00219749
CCAGTCA528000.060.6225126
GTAATCT407400.060.5613637
TGTAATC434150.059.84495536
TCCAGTC536600.059.6958925
ACTCCAG536950.059.64820523
CTTGTAA533900.059.04908434
Produced by FastQC (version 0.11.5)