FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19162694
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1716040.8955108295315888TruSeq Adapter, Index 11 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG232190.1211677230769327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA223600.060.8948532
CGGCTAC222800.060.59474633
CGTATGC218000.059.95136344
GTATGCC223900.058.57465445
TATGCCG222100.058.5450846
CACGGCT232300.058.5232331
ATGCCGT216850.058.5084447
GGCTACA231100.057.9039634
GTCACGG238200.057.54493329
TCACGGC238600.057.18410530
TGCCGTC210400.056.64398648
TCGTATG232600.056.4904343
CTCGTAT232350.056.430742
GCCGTCT204100.056.19690749
GCTACAT241500.054.9725435
AGTCACG250750.054.93004628
CTACATC254750.051.6323136
CCGTCTT225900.051.0527750
CAGTCAC274300.050.35190627
TCTCGTA261700.049.8754641
Produced by FastQC (version 0.11.5)