FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20522787
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC5499862.6798796869060717TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC1041470.5074700624237829TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTGG982260.47861920508164896TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGG882740.4301267659212172TruSeq Adapter, Index 10 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGG490430.23896851826216392TruSeq Adapter, Index 10 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGGTGG437330.21309483940948176TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATGC404730.1972100572890027TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATGC402880.1963086202668283TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATGG361360.1760774499097028TruSeq Adapter, Index 10 (97% over 45bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG326820.15924737707407868No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTC277070.1350060301264151TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTTTGC270740.13192165372081288TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTGG264590.12892498470115193TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATGG232080.11308405627364353TruSeq Adapter, Index 10 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTTTGG219450.10692992136009598TruSeq Adapter, Index 10 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGTTGG218890.10665705393716748TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTTTGG214080.10431331767951399TruSeq Adapter, Index 10 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATGG211880.10324133851800928TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTGTGG210090.10236913729114862TruSeq Adapter, Index 10 (97% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC1944000.067.51231431
ACTAGCT1940900.067.402132
TAGCTTA1939250.067.1534334
CTAGCTT1947600.066.931233
GCTTATC1905600.066.8778836
TCACTAG1969600.066.8513230
CAGTCAC1989550.066.3874227
CCAGTCA1990600.066.2272426
AGCTTAT1953950.066.20495635
ACTCCAG1991900.066.1640123
ACGTCTG2010200.066.074315
TCCAGTC1995500.066.0553725
GAACTCC2002150.066.0310321
CGTCTGA2014600.065.92461416
CGTATGC727950.065.9049844
ACACGTC2020800.065.8892613
CACGTCT2020900.065.77705414
TATCGCG493950.065.74439
GTATGCC760650.065.6944145
TATGCCG726250.065.6830246
Produced by FastQC (version 0.11.5)