FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19494627
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC275311414.122424604482045TruSeq Adapter, Index 9 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG448520.23007365054996948No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC291230.1493898806065897TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGC268940.13795596089117274TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3054750.068.48340644
TATGCCG3057300.068.3976646
GTATGCC3062500.068.3649645
ATGCCGT3042950.068.3564947
TCGTATG3063250.068.3321343
CTCGTAT3021100.068.2869942
AGTCACG3093550.068.2522528
TGCCGTC3011600.068.2305548
GCCGTCT2974500.068.1798649
CACGATC3081550.068.0537331
GTCACGA3092600.068.0423229
GATCTCG2971650.068.0279239
CGATCAG3074150.068.0164433
CCGTCTT2990300.067.858650
CAGATCT2977450.067.856837
CAGTCAC3115350.067.841127
CCAGTCA3109950.067.8072126
ACTCCAG3110900.067.7919423
AGATCTC2981200.067.7771338
ACGTCTG3138200.067.734415
Produced by FastQC (version 0.11.5)