FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23420096
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC3427171.4633458376942605TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTGG1163820.4969322072804484TruSeq Adapter, Index 8 (97% over 42bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1059160.4522440898619715No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGG723970.30912341264527693TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC688710.2940679662457404TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGGTGG544580.23252680091490657TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC401980.17163892069443268TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGG391310.16708300427120368TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGC377100.16101556543576936TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGG367540.15693360095535047TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTGG300530.1283214210565149TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGTTGG281400.12015322225835454TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGG270010.11528987754789732TruSeq Adapter, Index 8 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC1656400.066.0989529
CAGTCAC1684300.065.6234627
CCAGTCA1690100.065.3129226
AGTCACA1691400.065.03953628
ACTCCAG1702650.064.8716123
TCCAGTC1703500.064.8024425
ACACTTG1693700.064.38782532
GAACTCC1718700.064.3720621
TCACACT1702850.064.29796630
GCGGGGT358500.064.212150
CACTTGA1698000.063.99100533
ACGTCTG1739500.063.96407315
CTCCAGT1727100.063.96178424
CGTCTGA1741750.063.8933616
CGTATGC473100.063.6328844
CACACTT1722150.063.51546531
TATGCCG473300.063.324746
GTATGCC499300.063.26612545
ACACGTC1765050.063.22931313
CACGTCT1762350.063.2188214
Produced by FastQC (version 0.11.5)