FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16313831
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC6867634.209697893768791TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGG995280.6100835542552819TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC840310.5150905388194839TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTGG737000.4517639051183011TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGGTGG519710.31857017520899905TruSeq Adapter, Index 7 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGG377180.23120259122458728TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTGC358130.2195253830936461TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATGC338770.20765815215322506TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTGG330910.20284015446770293TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTGTGG255280.15648071872265934TruSeq Adapter, Index 7 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGATGC249950.15321355235321488TruSeq Adapter, Index 7 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGATGG239030.14651984564508483TruSeq Adapter, Index 7 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTTTGG238150.14598042605688388TruSeq Adapter, Index 7 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGTTGG234200.14355916767802732TruSeq Adapter, Index 7 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTGTGG230080.1410337032423592TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATGG214170.13128124227840782TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGGTGG182360.11178245011855278TruSeq Adapter, Index 7 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTGG175370.10749774225318383TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTC163930.10048528760657138TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC2000550.067.34106428
ACGTCTG2026400.067.0654215
ACACGTC2034550.066.968313
CGTCTGA2031050.066.9271816
ACCAGAT1997600.066.91749632
CAGTCAC2018650.066.9160427
GTCACCA2010100.066.91202529
GAACTCC2013050.066.9086621
ACTCCAG2008200.066.8908523
CACGTCT2035000.066.8638514
GCACACG2039350.066.8621611
CCAGTCA2012300.066.84624526
CACACGT2040700.066.8453712
TCCAGTC2018450.066.7014225
CCAGATC1991850.066.5887533
CGTATGC871250.066.4575344
CTCGTAT912100.066.3991242
CACCAGA2017600.066.3896931
TCACCAG2026500.066.35609430
TATGCCG862550.066.28767446
Produced by FastQC (version 0.11.5)