FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_AKM13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_AKM13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18194227
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC10889085.98490938911557TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC1263350.067.73685529
CGTATGC1184650.067.5094644
TATGCCG1178600.067.41907546
GTATGCC1189000.067.4035945
ATGCCGT1142500.067.2969747
TCACGCC1266700.067.1953530
CACGCCA1265400.067.1616731
AGTCACG1284350.067.0760928
CTCGTAT1207100.067.0502442
TCGTATG1205050.067.0159843
TGCCGTC1079350.066.6453348
GCCGTCT1038200.066.4209149
ACGCCAA1285550.066.10660632
CGCCAAT1280700.066.09930433
GCCAATA1280000.066.02952634
CCAGTCA1303350.065.99116526
CAGTCAC1306750.065.97500627
TATCTCG1262400.065.9484739
TCTCGTA1237900.065.6369941
ACGTCTG1326000.065.6058515
Produced by FastQC (version 0.11.5)