FastQCFastQC Report
Wed 26 Jan 2022
HFL7VAFX3_n01_7797-in.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFL7VAFX3_n01_7797-in.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4746518
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC813621.7141407659256744TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTTTGC125180.26373017020055545TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGG74180.1562829847058412TruSeq Adapter, Index 6 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTTTGG68870.1450958365690386TruSeq Adapter, Index 6 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATTC57090.12027764352731835TruSeq Adapter, Index 6 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)