FastQCFastQC Report
Wed 26 Jan 2022
HFL7VAFX3_n01_5184-chp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFL7VAFX3_n01_5184-chp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5827
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC149825.707911446713577TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGC1833.140552599965677TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGG1292.2138321606315428TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGG981.6818259824952806TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTC661.1326583147417195TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGG450.7722670327784451TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTTG380.6521366054573537TruSeq Adapter, Index 4 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC320.549167667753561TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATGTCGTATGC310.5320061781362623TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGC290.4976831989016646TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTG260.4461987300497683TruSeq Adapter, Index 4 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAGCTCGTATGC220.3775527715805732TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTTC220.3775527715805732TruSeq Adapter, Index 4 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGTTGG190.3260683027286769TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGC180.3089068131113781TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATGC180.3089068131113781TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCACTCTCGTATGC180.3089068131113781TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTGTGC170.2917453234940793TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTTG160.2745838338767805TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGG160.2745838338767805TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGTGG150.25742234425948174TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTTTGG140.24026085464218294TruSeq Adapter, Index 4 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTTTGC110.1887763857902866TruSeq Adapter, Index 4 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAAGC100.1716148961729878TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTTC80.13729191693839024TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTGTGG70.12013042732109147TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGTTTG70.12013042732109147TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGTTGC70.12013042732109147TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTTTGC70.12013042732109147TruSeq Adapter, Index 4 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGGTGG70.12013042732109147TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGA60.10296893770379267TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATTC60.10296893770379267TruSeq Adapter, Index 4 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGG60.10296893770379267TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATGG60.10296893770379267TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATGTCGTATGG60.10296893770379267TruSeq Adapter, Index 4 (97% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)