FastQCFastQC Report
Wed 26 Jan 2022
HFL7VAFX3_n01_11617-chp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFL7VAFX3_n01_11617-chp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753111
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGGTGG29440.16793003979782228TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGTTGG20080.11453923910123205TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGTTGG18760.10700976720812316TruSeq Adapter, Index 5 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)