FastQCFastQC Report
Sun 18 Nov 2018
HFL77BGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFL77BGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18297237
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2309111.2619992843728263No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA346550.18940018102186687No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA216900.11854248813632352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA39750.065.768181
GTCCAAT29550.063.2647861
ACCTGGC64100.047.946024
CACCTGG72750.042.0092743
CCTGGCC76450.041.4647335
TCAAAGG56650.040.6595737
TCCAATC51750.039.250622
GCCCGGA80100.038.796179
GGGCACT6400.037.240531
CCGGCAT14750.036.861811
TGGCCCG85500.036.8518037
CTGGCCC86400.036.495676
GACATCG117200.036.3682983
CTAGCAT65750.034.5501481
CTCCTGG86550.033.3335726
CCCGGGT10200.032.8592871
GAGCGCT47600.032.1804053
TCCTGGT91550.031.6473437
CCTGGTG91700.030.8848448
TCCACTC92150.030.762662
Produced by FastQC (version 0.11.5)