FastQCFastQC Report
Sun 18 Nov 2018
HFL77BGX9_n02_C1aD9_K11777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFL77BGX9_n02_C1aD9_K11777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23168550
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA692610.29894404267854485No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT542480.23414499396811625No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC506430.2185851078293635No Hit
CGCCTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACG492720.2126676032811721No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG462720.19971901564836816No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG405740.17512533153779586No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC397120.1714047706913035No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG395290.17061490684570246No Hit
CTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACGTTT383920.16570739213286978No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC363800.15702320602713593No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT342180.14769159053976189No Hit
CGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAG338690.14618523817847903No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA336280.1451450349719771No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC331340.14301283420844205No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC330390.14260279560006991No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA326890.14109212704290947No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT325220.14037132233135005No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC323500.13962893664040263No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA313540.1353300055463117No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT312760.1349933422678588No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT309870.13374596165923203No Hit
AAATAAAGCAGTTATATTCGGTAAAGCGAATGATTAGAGGAATTGTGGGA307170.13258058877227968No Hit
GCTAAATTGATTGGTTGAATACCGATAAGTTTTGTTAACTCTATCCGTTT302820.1307030435655231No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA299560.12929596370942506No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA287300.12400430756348585No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC285420.12319286273849679No Hit
GTTGAAAGCACGACATCTCGTCCGATCTGTCAAGTTAAGCAACGTTGGGC282330.12185915821231799No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT270390.11670562033446201No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG268490.11588554311771776No Hit
GGGAAACTTCAGAGGGAACCAGCTACTAGATAGTTCGATTAGTCTTTCGC267450.11543665874644722No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT260970.11263976381776157No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC258730.11167293594117889No Hit
CGGGGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCG244780.10565184269192504No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG244170.10538855474339136No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG240760.10391673194912932No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT237880.10267366753638014No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG232480.10034292176247543No Hit
GTGAACTATACCTGAGCAGGATGAAGCCAGAGGAAACTCTGGTGAAAGTC231840.10006668522630895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT55800.082.351261
TCCAATC59200.078.902262
CTTGTTA81900.068.162141
GGGTCTG56000.063.7239042
GTTACGA89650.061.517624
TTACGAC91600.060.0509575
TCAAAGG86700.059.774757
CAAAGGA84050.058.643628
GGTCTGG61300.058.5292173
GCGCCTT29050.057.9752431
GCGAGCG113250.057.88291
TGGGTCT51600.057.5405921
TGAAAGC78600.056.0672233
GGGCACT14250.052.9808851
AAGCACG84800.051.7917676
ATCAAAG116850.051.78956
CCGGCAT31150.051.2702451
GCACGAC85700.051.0864458
ACCTGGC167700.050.7341274
CACGACA87100.050.434479
Produced by FastQC (version 0.11.5)