FastQCFastQC Report
Sun 18 Nov 2018
HFL77BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFL77BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18297237
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1370740.7491513609404523No Hit
TGTGAAGTGAGTACGGTTTGATCCATGCAGTGTAACATTAAAATGAAAAC297470.16257645894841938No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA296330.16195341405918282No Hit
GTTTGATCCATGCAGTGTAACATTAAAATGAAAACATTAAAAAAAAAAAA246770.13486735729553045No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA229670.12552168395698213No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA201460.11010405560140037No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG199780.10918588418568334No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT195890.10705988013381473No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA191350.1045786311889604No Hit
TAACAACTCACCTGCCAAATCAACTAGCCCTGAAAATGGATGGCGCTTGA183050.10004242717083459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT25950.077.379561
CTTGTTA37700.065.658241
CCGGCAT13800.053.969951
TCCAATC40850.051.551812
CACCTGG68550.047.8092463
TCAAAGG51800.047.583777
ACCTGGC68950.046.5813874
GAGCGCT47750.046.0097853
GGCATCG13400.045.993353
GACATCG131600.043.030473
CCTGGCC81900.039.5682075
CTAGCAT64450.037.160531
GCCCGGA88200.036.6581089
GCTTGTA61300.035.7154547
CGCAGGC67050.035.4149742
CGGGTCT9150.034.3145521
TCTGGAG35150.033.2065165
GGCTCCA76100.033.0575876
CTTGTAA67600.032.7088478
AGCGCTT66950.032.703884