FastQCFastQC Report
Sun 18 Nov 2018
HFL77BGX9_n01_T1aD6_K11777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFL77BGX9_n01_T1aD6_K11777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31702657
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1146980.3617930194305165No Hit
CTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACGTTT883240.278601254147247No Hit
CGCCTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACG757330.23888534011518342No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG755440.2382891755728865No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA703790.2219971657265194No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG698660.2203790048259993No Hit
TTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGAGAGGA653920.20626662301522553No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC648400.20452544403454892No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC627470.1979234737328168No Hit
CGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAG606070.19117325087294734No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT578020.1823254120309222No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA568350.17927519450499055No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG554830.17501056772623189No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT554510.17490962981430863No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC551210.1738687075975998No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC549550.17334509217949776No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC542050.17097935986879587No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT540290.1704242013532178No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT521680.16455403091292947No Hit
AAATAAAGCAGTTATATTCGGTAAAGCGAATGATTAGAGGAATTGTGGGA517840.16334277596985008No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG501070.15805299852312063No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC472310.1489812036890157No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG453730.14312049617797018No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT452740.14280821951295755No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA440550.13896311593063004No Hit
CTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA438430.1382944022641383No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA438280.13824708761792426No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC428960.1353072709331587No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA421620.13299200757841842No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG418200.13191323364473836No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG418000.1318501474497863No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG412800.13020990638103297No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG412490.1301121227788573No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG403150.12716599747459653No Hit
GCTAAATTGATTGGTTGAATACCGATAAGTTTTGTTAACTCTATCCGTTT399500.12601467441672162No Hit
GACTAATCGAACTATCTAGTAGCTGGTTCCCTCTGAAGTTTCCCTCAGGA395810.12485073411985627No Hit
GGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGT393920.12425456957755938No Hit
GAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTT389030.12271211211098174No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT388390.12251023628713517No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG376690.1188196938824402No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC366210.11551397726695274No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG362350.11429641370437815No Hit
GCCTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACGT361290.11396205687113228No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC360410.11368447761334326No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC358990.1132365656291837No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA352450.11117364705425163No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT347560.109631189587674No Hit
TGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCACTGT346760.10937884480786578No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG341490.10771652357087924No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT341230.10763451151744158No Hit
GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA340600.10743579000334262No Hit
AGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGA335260.10575138859812286No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA331700.10462845432797636No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT325170.1025686900627919No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT324640.10240151164616895No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG320360.10105146707419509No Hit
GAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTG320330.10104200414495226No Hit
GTGAACTATACCTGAGCAGGATGAAGCCAGAGGAAACTCTGGTGAAAGTC319290.1007139559312016No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATAGTTCGATTAGTCTTTCGCC319020.10062878956801632No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT63400.0109.092761
TCCAATC68900.0100.701912
CCGGCAT26450.078.941181
CTTGTTA104050.078.526931
TCAAAGG102900.075.671417
CAAAGGA102750.072.1126258
GCGAGCG147250.070.013381
GCGCCTT33550.068.718141
ATCAAAG113750.068.455256
GGGTCTG82600.067.849152
GGTCTGG83650.067.084063
TGGGTCT69150.066.261561
CGGGTCT21450.065.9089051
GTTACGA124000.065.777684
TTACGAC128600.063.594165
TGTGGCG38100.056.3262753
CGATGGG23900.054.9057041
GGCATCG30600.054.731193
CGGCATC31950.054.234052
CTAGCAT195450.054.045641
Produced by FastQC (version 0.11.5)