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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2018-12-12, 21:12 based on data in: /beegfs/mk5636/logs/html/HFL77BGX9/merged


        General Statistics

        Showing 34/34 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HFL77BGX9_n01_C1aD5_K11777
        91.8%
        45%
        33.4
        HFL77BGX9_n01_C1aD6_K11777
        93.2%
        45%
        27.1
        HFL77BGX9_n01_C1aD9_K11777
        90.0%
        45%
        23.2
        HFL77BGX9_n01_C1bD5_K11777
        92.8%
        45%
        28.9
        HFL77BGX9_n01_C1bD6_K11777
        93.1%
        45%
        26.5
        HFL77BGX9_n01_C1bD9_K11777
        92.2%
        45%
        24.8
        HFL77BGX9_n01_C1cD6_K11777
        93.9%
        45%
        28.8
        HFL77BGX9_n01_C1cD9_K11777
        94.9%
        45%
        28.4
        HFL77BGX9_n01_T1aD5_K11777
        91.7%
        44%
        25.6
        HFL77BGX9_n01_T1aD6_K11777
        93.7%
        45%
        31.7
        HFL77BGX9_n01_T1bD5_K11777
        92.0%
        45%
        24.3
        HFL77BGX9_n01_T1bD6_K11777
        92.3%
        45%
        27.8
        HFL77BGX9_n01_T1bD9_K11777
        90.2%
        44%
        28.2
        HFL77BGX9_n01_T1cD5_K11777
        91.2%
        45%
        26.8
        HFL77BGX9_n01_T1cD6_K11777
        93.0%
        45%
        23.9
        HFL77BGX9_n01_T1cD9_K11777
        95.1%
        45%
        130.5
        HFL77BGX9_n01_undetermined
        81.3%
        44%
        18.3
        HFL77BGX9_n02_C1aD5_K11777
        89.0%
        44%
        33.4
        HFL77BGX9_n02_C1aD6_K11777
        88.9%
        45%
        27.1
        HFL77BGX9_n02_C1aD9_K11777
        87.2%
        45%
        23.2
        HFL77BGX9_n02_C1bD5_K11777
        89.8%
        44%
        28.9
        HFL77BGX9_n02_C1bD6_K11777
        89.7%
        45%
        26.5
        HFL77BGX9_n02_C1bD9_K11777
        89.7%
        45%
        24.8
        HFL77BGX9_n02_C1cD6_K11777
        91.5%
        45%
        28.8
        HFL77BGX9_n02_C1cD9_K11777
        91.9%
        45%
        28.4
        HFL77BGX9_n02_T1aD5_K11777
        89.1%
        44%
        25.6
        HFL77BGX9_n02_T1aD6_K11777
        90.0%
        45%
        31.7
        HFL77BGX9_n02_T1bD5_K11777
        88.9%
        45%
        24.3
        HFL77BGX9_n02_T1bD6_K11777
        89.6%
        45%
        27.8
        HFL77BGX9_n02_T1bD9_K11777
        87.0%
        44%
        28.2
        HFL77BGX9_n02_T1cD5_K11777
        88.2%
        45%
        26.8
        HFL77BGX9_n02_T1cD6_K11777
        89.8%
        45%
        23.9
        HFL77BGX9_n02_T1cD9_K11777
        92.2%
        45%
        130.5
        HFL77BGX9_n02_undetermined
        64.5%
        43%
        18.3

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 17/17 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        18,297,237
        3.3
        C1aD5_K11777
        33,408,494
        6.0
        C1bD5_K11777
        28,904,418
        5.2
        T1aD5_K11777
        25,643,762
        4.6
        T1bD5_K11777
        24,310,469
        4.4
        T1cD5_K11777
        26,794,842
        4.8
        C1aD6_K11777
        27,112,576
        4.9
        C1bD6_K11777
        26,521,987
        4.8
        C1cD6_K11777
        28,808,236
        5.2
        T1aD6_K11777
        31,702,657
        5.7
        T1bD6_K11777
        27,847,014
        5.0
        T1cD6_K11777
        23,944,972
        4.3
        C1aD9_K11777
        23,168,550
        4.1
        C1bD9_K11777
        24,816,173
        4.4
        C1cD9_K11777
        28,402,401
        5.1
        T1bD9_K11777
        28,215,537
        5.1
        T1cD9_K11777
        130,492,099
        23.4

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        4.0
        626,118,784
        558,391,424
        3.3
        1.1

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (151bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

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        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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