FastQCFastQC Report
Wed 12 Dec 2018
HFL77BGX9_n02_T1cD6_K11777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFL77BGX9_n02_T1cD6_K11777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23944972
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG936160.39096307984824535No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT777630.3247571139360697No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG739030.30863681945420524No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG718780.30017992921436704No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA702920.2935564092536838No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG690620.2884196314783747No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC689050.2877639614696564No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG682580.28506193283500186No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA610020.2547591202027716No Hit
CTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACGTTT577520.24118633339809295No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC561830.23463380955300342No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG558600.23328488335672307No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA545160.22767201398272674No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC519880.21711447396973363No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT514550.2148885369337663No Hit
CGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAG474420.19812927741155845No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA448220.1871875231259406No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC429200.17924431066363328No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT427910.17870557543353988No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG426040.17792461816200913No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC419410.17515576965385468No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA415970.1737191423736056No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG415370.1734685678479808No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT413790.1728087215971687No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC412630.17232427751429402No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC406080.16958883894288956No Hit
CGCCTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACG405620.1693967318065772No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA398030.166226964057423No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT397970.16620190660486053No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT395180.16503673506070501No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC388930.16242658375211297No Hit
CTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA386830.16154957291242603No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA368670.15396551727018098No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG352980.14741299342509148No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG345620.1443392792440935No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA343740.143554145730469No Hit
CTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTC324110.1353561825004431No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC323110.13493855829106838No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT320360.13379009171528788No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC310210.1295512059901344No Hit
GTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACT308400.12879530617116614No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC308310.1287577199923224No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT303430.12671971385057373No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA302150.1261851548625741No Hit
CTCAGATCAGAAAACCAACAAAATAGAACCGAGATCCTATTTCATTATTC301790.12603481014719917No Hit
CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA298160.12451883426716892No Hit
CTTCGAACCTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAA296890.123988451521263No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT289870.12105672957145241No Hit
CTGAATACTCGTGCATGGAATAATGAAATAGGATCTCGGTTCTATTTTGT281120.11740251773942353No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG280440.11711853327704873No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT276720.11556497121817473No Hit
CAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTT276450.11545221268164357No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG274470.1146253167470816No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT272460.1137858920862384No Hit
AAATAAAGCAGTTATATTCGGTAAAGCGAATGATTAGAGGAATTGTGGGA271120.11322627564567626No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG269790.11267083544720788No Hit
ATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTA267680.1117896483654272No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC265270.11078317402083411No Hit
ATTTTATCGTCCAGACAACTTCTTAGAGGGACAAGCGGTGTTTAGCCGCA262350.1095637113294599No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA262050.10943842406664747No Hit
GGTAATTCCAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGC257880.10769693111355487No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA256700.10720413454649268No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC256110.1069577362629616No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG255080.10652758332730564No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA252590.10548769904596256No Hit
CTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACT243970.1018877783611524No Hit
CTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACAACTTC243700.10177501982462121No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCTCATAGATCCTC243610.1017374336457775No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT243140.10154115026737137No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG242390.10122793211034033No Hit
ATTTAAACCTGTTAACGAGGATCTATGAGAGGGCAAGTCTGGTGCCAGCA242380.10122375586824658No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT239730.10011705171340354No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT57850.0103.509181
TCCAATC61300.0100.5705262
CTTGTTA116050.095.566671
CGGGGTC10600.081.4834441
GTTACGA136350.080.94884
TTACGAC139050.079.587125
CTAGCAT136850.075.8437041
TCAAAGG89200.074.429697
GCGCCTT16700.073.8857651
AGAACCC27350.073.66917145
GGGTCTG33650.071.12812
GCGAGCG54700.069.131931
GGTCTGG35150.067.6348343
ACCTGGC193050.065.244764
CACCTGG193000.065.1953
TGGGTCT30350.065.048681
ATCAAAG105350.064.391666
CAAAGGA98500.064.388368
TCGTGTT43550.064.23881145
TACGCAA163200.063.382499
Produced by FastQC (version 0.11.5)