FastQCFastQC Report
Wed 12 Dec 2018
HFL77BGX9_n01_T1cD9_K11777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFL77BGX9_n01_T1cD9_K11777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130492099
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA4843820.37119642009896703No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG3596220.27558909907641227No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG3279220.2512964405607423No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2827480.21667825268103014No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT2505610.19201239149352636No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG2460080.18852329136034512No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC2300870.1763225526780744No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG2277090.17450022012443833No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA2212210.16952827159290312No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA2204480.16893589856348315No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT2119750.16244278513751242No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC2085210.15979588158820252No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG2068040.15848009311276387No Hit
CGCCTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACG2043580.15660565012445696No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2040770.15639031141647894No Hit
TTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGAGAGGA2029560.15553125557433173No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA1994460.15284143754941054No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA1982320.1519111130245518No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC1942860.14888717515379993No Hit
AAATAAAGCAGTTATATTCGGTAAAGCGAATGATTAGAGGAATTGTGGGA1849190.141708962777892No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG1798400.13781677310593343No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA1773480.13590707894123152No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT1751420.13421655513411582No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1724230.13213290407720393No Hit
CGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAG1693760.1297978968059974No Hit
TGGGTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCG1685300.12914958169229848No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1668980.12789893126019836No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA1662990.12743989963714203No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC1658050.1270613326558568No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT1626210.12462133818538698No Hit
CTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACGTTT1613290.12363123992664107No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC1607500.12318753490201734No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG1546380.11850372642101495No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1540770.11807381533498054No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG1537320.11780943151201821No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT1525830.11692891843206538No Hit
TGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCC1485500.11383830985813172No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC1447690.11094081642444881No Hit
GAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTG1435710.11002275317833611No Hit
CTGGCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGG1430770.1096441861970509No Hit
CTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA1396460.10701490823593848No Hit
GGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGA1395020.10690455672722377No Hit
GACTAATCGAACTATCTAGTAGCTGGTTCCCTCTGAAGTTTCCCTCAGGA1382420.10593898102597No Hit
GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC1381680.10588227261176938No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA1378820.10566310225418322No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA1351690.10358404917680111No Hit
GGAGTCTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGA1348650.10335108488062561No Hit
TGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCACTGT1341430.10279779467720876No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT1335160.10231730581634678No Hit
GTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCA1318660.10105286144565734No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT333650.0102.432311
TCCAATC362500.095.442122
CCGGCAT121200.076.029381
TCAAAGG501700.075.9115147
CTTGTTA476400.075.285041
CAAAGGA499400.073.9963768
GGGTCTG466800.070.560172
ATCAAAG570700.069.695276
GGTCTGG489000.067.772043
CGATGGG142650.067.697241
CGGGTCT97600.067.597411
GTTACGA537050.066.852424
TTACGAC542750.066.177325
TGGGTCT401200.065.12721
GGCATCG116300.065.0208743
GGGCACT53200.064.459671
GCGAGCG877200.062.7805141
CGGCATC124750.061.9532852
TGTGGCG219400.059.7792053
GCGCCTT100400.059.5742761
Produced by FastQC (version 0.11.5)