FastQCFastQC Report
Wed 12 Dec 2018
HFL77BGX9_n01_T1cD5_K11777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFL77BGX9_n01_T1cD5_K11777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26794842
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1422580.530915614281286No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA617660.23051451469652257No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG596590.22265106097658646No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA589850.22013565148098277No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT569200.21242894434682613No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT528040.19706777894043936No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA523760.1954704565901154No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA493440.18415484592146503No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT493190.1840615443823106No Hit
GGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGA473180.17659368918838933No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC468370.17479856757505793No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG464870.17349234602689578No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG463130.17284296731438087No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGCGGGGAAAGA457790.17085004643804208No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC456510.17037234255757133No Hit
GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC454220.1695177004589167No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT450950.1682973163267766No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT449240.16765913379896027No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG427400.1595083113384285No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT392150.14635279431765263No Hit
CAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGG391920.14626695690163055No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC389600.14540111861827734No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA388620.14503537658479196No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC383660.14318427404796788No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGCGGGGAAAGAA371400.13860876656783422No Hit
GTGGTATTTCACTGTTGGTATTTGTATAATAAATACAAAAAAAACCTCCC369660.13795938785531933No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT368500.1375264687136427No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG367840.13728015265027502No Hit
GACTAATCGAACTATCTAGTAGCTGGTTCCCTCTGAAGTTTCCCTCAGGA365020.13622771128861294No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG352030.1313797633141483No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG349800.1305475135848907No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA341310.12737899331520597No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT331200.12360587907180047No Hit
GTTAAATAAAGCAGTTATATTCGGTAAAGCGAATGATTAGAGGAATTGTG326790.12196003992111616No Hit
CAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGTTC325740.12156817345666752No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG317670.11855639977276222No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA316840.1182466386627695No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC307980.11494003211513619No Hit
CTGGCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGG305580.11404433733925358No Hit
GCGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCA303160.1131411784402386No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA299040.11160356907497346No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC295020.11010328032537008No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC294320.10984203601573765No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT290000.10822978541914896No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG287360.10724452116567809No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA286730.1070094012870089No Hit
CGAATAAGTAAAGAATCGATGAAAGTAGTGGTATTTCACTGTTGGTATTT285830.10667351574605291No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG282280.1053486338900599No Hit
GCCACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGC279250.10421781923550807No Hit
GGCTTGGCAAACACAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAG274060.10228087928266193No Hit
GGAGTCTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGA272980.10187781663351476No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA272250.10160537613918381No Hit
GCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCAG270590.10098585391919834No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT269040.1004073843764408No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGC77450.091.7366941
ACCTGGC250450.085.3418354
CACCTGG260350.082.234813
GGCCGAC38550.081.621361
CCTGGCC270600.079.5229345
GTCCAAT100550.075.492371
CGTCCAA43900.073.490971
TCCAATC105450.072.055072
CTGGCCC303750.070.940276
CCTTGGG19650.070.837524145
GGGCACT11600.069.375081
TGGCCCG312100.069.272317
GCCCGGA316300.068.188719
CGGGGTC11900.063.3614121
GCGATGG34650.062.5613981
GCCGCAG135350.061.974571
CAAAGGA139100.060.5650838
TCAAAGG142550.060.523717
CGCTACT23650.058.2453081
GCGAGCG121900.057.9896852
Produced by FastQC (version 0.11.5)