FastQCFastQC Report
Wed 12 Dec 2018
HFL77BGX9_n01_T1bD5_K11777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFL77BGX9_n01_T1bD5_K11777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24310469
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1130700.4651082626172288No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT625740.25739528102069936No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC585580.24087564908764203No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC518480.21327437162976987No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC484160.19915699693000577No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG481230.19795175485919253No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT471350.19388766214259381No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA444220.18272786098861357No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC436240.17944532456366843No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG425300.17494520570541028No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG418180.17201642633879255No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT417910.17190536307629442No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA415930.17109089915130804No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG409350.16838424630968657No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC406150.16706794097637526No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG401180.16502355425557608No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC396370.16304498280144245No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT375340.15439438868908698No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC368800.15170418966413193No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT366690.15083625083497978No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT362840.14925257098083955No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT362500.14911271353917524No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA350990.14437812779342102No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT348350.14329217589343918No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT346750.14263402322678348No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG315470.12976713859366515No Hit
AAATAAAGCAGTTATATTCGGTAAAGCGAATGATTAGAGGAATTGTGGGA309890.12747183116870348No Hit
CGCCTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACG307290.12640233308538804No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA305180.12553439425623586No Hit
TAAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAA303780.12495851067291215No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA301380.12397128167292865No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA297460.12235880763962226No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT286890.11801088658552822No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC286450.11782989460219792No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT285940.11762010843970143No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG285510.11744322991053771No Hit
ATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTA283960.11680564451471505No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA278360.1145021101814202No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC278350.1144979967272536No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG276230.11362594444393483No Hit
GCTGATTCCTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCAT274870.11306651467727752No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA271140.11153219627313647No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTCTGTGAAGAGTCATGA266970.10981688588566514No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG263120.10823320603152495No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC260160.10701562359821196No Hit
CGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAG259400.10670300108155051No Hit
CTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCT258200.10620938658155875No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT257400.10588031024823091No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG256480.10550187246490392No Hit
GACTAATCGAACTATCTAGTAGCTGGTTCCCTCTGAAGTTTCCCTCAGGA255220.10498357723991256No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT254880.10484371979824823No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA252510.10386883116076454No Hit
CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG250610.1030872748691109No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT249250.1025278451024536No Hit
GACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTG245360.100927711431647No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT70450.0100.336541
TCCAATC74150.095.8211062
CCGGCAT25500.080.744631
CAAAGGA100350.077.016118
GGGCACT9200.076.439771
TCAAAGG110050.072.9945767
CTTGTTA83850.072.8886641
ATCAAAG108650.071.9353566
GCGCCTT18200.070.906181
GGCATCG24050.069.63753
GGGTCTG61000.069.4111562
GCGAGCG97600.069.2311551
CGGCATC25350.069.212352
TGGGTCT52850.066.532261
GGTCTGG64450.066.1455463
GTTACGA93000.065.95344
TTACGAC96350.063.6605265
CCTTGGG13300.060.50655145
CGATGGG19700.059.2509271
CGGGTCT13250.056.905331
Produced by FastQC (version 0.11.5)