FastQCFastQC Report
Wed 21 Nov 2018
HFKYCBGX9_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKYCBGX9_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349582383
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGT12097150.043.9830741
TCCACGA258667650.043.8292057
CCACGAG259328200.043.7947048
GGTCTCT258929150.043.78606814
AGGTCTC259634350.043.78040313
GAGGTCT259524800.043.77690512
ACGAGGT259288050.043.773510
CACGAGG259333150.043.7618269
AACGTCC12010300.043.7470633
GGTCCAC96390100.043.7204325
GTCTCTT51404700.043.70980515
GTCTCTA78644150.043.7034815
GGGTCCA12437900.043.6953934
CGGTCCA14718850.043.6881754
ACTGTCC14089300.043.687753
GTCCACG259483550.043.6817056
CGTCCAC70001300.043.6814085
AATGGTC14028150.043.6456032
ACGTCCA12493900.043.641834
GTCTCTG66958900.043.63213715
Produced by FastQC (version 0.11.5)