FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14945971
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11479407.6805983365015225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGG5300.044.7941744
GGGGAGG8550.031.0456161
AGGGGGG8550.029.8089545
CTAGCAG8750.027.9157228
CTGACTC9900.025.393222
GCTGACT11150.022.2400761
TAGCAGA12350.020.6260579
ACTCTAG13900.020.0939435
CTCTAGC12850.019.2905756
GCACACG16800.018.90133311
TCGGAAG20050.018.6323073
CGCTATG3850.018.1998529
TCTATCG13250.017.91425316
CACGTCT16950.017.91184614
CACACGT16950.017.9112512
ACGTCTG16650.017.81867815
AGCACAC17700.017.34881210
CTAGCAT13800.017.256726
CTATCGA14500.017.09486817
ACACGTC18000.016.86659613
Produced by FastQC (version 0.11.5)