FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_084.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_084.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3226747
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCTCGTATGC132920.41193189301795274TruSeq Adapter, Index 6 (97% over 36bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT57880.17937569942731796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52260.16195877767919206No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA43280.1341288920389482No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG42340.13121574142627235No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT41980.13010006672354543No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG35960.11144350641683404No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC35380.10964603050688511No Hit
GGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATG32600.10103054252471606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22600.050.32567644
TATGCCG22850.050.23608446
ACGTCTG24650.049.83083315
GTATGCC23100.049.389445
TCGTATG22750.048.91706543
CTCGTAT23250.047.41499742
GCACACG25950.047.20036311
CACGTCT26250.046.6602114
ACTCCCG25750.046.2122132
AGCACAC26900.045.66353210
CCCGAAT26100.045.591835
CACTCCC26050.045.5463731
CACACGT26900.045.53273412
GTCACTC26100.045.3257229
CAGTCAC26350.045.16134327
AGTCACT27250.044.05441328
ACACGTC28500.043.099313
CCAGTCA27950.042.70063826
GCCGTCT25900.042.15894349
GAGCACA29750.041.4066779