Basic Statistics
Measure | Value |
---|---|
Filename | HFKW7BGX9_n01_NCR_084.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3226747 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCTCGTATGC | 13292 | 0.41193189301795274 | TruSeq Adapter, Index 6 (97% over 36bp) |
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT | 5788 | 0.17937569942731796 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 5226 | 0.16195877767919206 | No Hit |
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA | 4328 | 0.1341288920389482 | No Hit |
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG | 4234 | 0.13121574142627235 | No Hit |
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT | 4198 | 0.13010006672354543 | No Hit |
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG | 3596 | 0.11144350641683404 | No Hit |
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC | 3538 | 0.10964603050688511 | No Hit |
GGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATG | 3260 | 0.10103054252471606 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 2260 | 0.0 | 50.325676 | 44 |
TATGCCG | 2285 | 0.0 | 50.236084 | 46 |
ACGTCTG | 2465 | 0.0 | 49.830833 | 15 |
GTATGCC | 2310 | 0.0 | 49.3894 | 45 |
TCGTATG | 2275 | 0.0 | 48.917065 | 43 |
CTCGTAT | 2325 | 0.0 | 47.414997 | 42 |
GCACACG | 2595 | 0.0 | 47.200363 | 11 |
CACGTCT | 2625 | 0.0 | 46.66021 | 14 |
ACTCCCG | 2575 | 0.0 | 46.21221 | 32 |
AGCACAC | 2690 | 0.0 | 45.663532 | 10 |
CCCGAAT | 2610 | 0.0 | 45.5918 | 35 |
CACTCCC | 2605 | 0.0 | 45.54637 | 31 |
CACACGT | 2690 | 0.0 | 45.532734 | 12 |
GTCACTC | 2610 | 0.0 | 45.32572 | 29 |
CAGTCAC | 2635 | 0.0 | 45.161343 | 27 |
AGTCACT | 2725 | 0.0 | 44.054413 | 28 |
ACACGTC | 2850 | 0.0 | 43.0993 | 13 |
CCAGTCA | 2795 | 0.0 | 42.700638 | 26 |
GCCGTCT | 2590 | 0.0 | 42.158943 | 49 |
GAGCACA | 2975 | 0.0 | 41.406677 | 9 |