FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_083.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_083.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3212119
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA78180.24339073365588262No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT77290.2406199770307389No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG70420.21923222645238238No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT56380.1755227623883175No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA49710.15475765374819553No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG47570.1480953850090859No Hit
GGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATG45710.14230481498350467No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC44100.13729254738071658No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT42360.13187556251807606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42200.13137744896748843No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGC42140.1311906563860181No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT41740.129945372509549TruSeq Adapter, Index 13 (97% over 40bp)
GCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGC41690.12978971202499034No Hit
CCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCAC38170.1188312139120624No Hit
CCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTC37020.11525102276721379No Hit
GGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGG36870.11478404131353788No Hit
GCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTC36160.11257366243280525No Hit
GGGGTGTTCTGCTGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGAT36140.1125113982389818No Hit
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATC35310.10992743419530844No Hit
GCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAG34840.10846422564045727No Hit
CGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCG33560.10447931723575621No Hit
GTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGA32600.1014906359322304No Hit
CGCGCTTCTCGTTGGGGTCTTTGCTCAGGGCGGACTGGGTGCTCAGGTAG32230.1003387483464965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7550.039.86437648
GTATGCC8000.037.6220147
CGTATGC8650.035.1995146
CTCGTAT8900.033.02953344
GCACACG10200.031.9067411
ACGTCTG10150.031.71864715
CACACGT10700.030.41529812
TCGTATG9950.030.2488545
CACGTCT12100.026.60696614
AGCACAC12500.026.31585710
CTAGCAG15350.025.9893918
CTGACTC15650.025.9392152
GTCACAG11800.025.80624429
GCCGTCT12100.025.74135251
CAGTCAC12350.025.22262627
ACACGTC13300.024.73256313
CACAGTC12700.024.52827831
GCTGACT16750.024.03281
TCTCGTA12450.023.89293743
AGTCACA13450.023.42076128
Produced by FastQC (version 0.11.5)