FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_082.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_082.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6102302
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82690.1355062401041443No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT79680.13057367531138248No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA77080.12631298811497693No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG68090.11158084277048236No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT64740.10609111119049827No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC61280.10042111976758936TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC14150.033.8837144
GTATGCC14200.033.51849745
TATGCCG14300.033.0390946
TCGTATG16300.028.98497843
ACGTCTG17100.028.85549215
GCACACG17800.028.50712811
CTCGTAT17200.027.26597242
CACTATA18000.027.02809131
CACACGT20150.025.00880412
CTATAAT19500.024.76972233
CACGTCT21350.023.60315514
AGCACAC21600.023.49198510
TATCTCG20750.022.93889639
CTAGCAG22000.022.7467218
GCCGTCT22100.022.3276549
TATAATA23250.021.37676834
AGTCACT23550.021.10410528
ACTATAA24550.020.81490732
ACACGTC24950.020.1974913
CAGTCAC23750.020.18937727
Produced by FastQC (version 0.11.5)