FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_081.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_081.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4825983
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA81950.1698099641047223No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT81340.16854597291370482No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG80120.1660179905316699No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT60140.12461709873408174No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58910.12206839518498097No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA52820.10944920444187224No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG51120.10592660604067607No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC50020.10364727766343147No Hit
GGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATG48900.10132650695205515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8250.033.93581444
TATGCCG8650.031.96194546
GTATGCC9500.030.94410345
TCGTATG10400.026.58373343
CTCGTAT10300.025.82278642
ACGTCTG11350.025.59121715
GCACACG11250.024.8854911
CACACGT12700.022.31978612
GCCGTCT13200.022.27014749
CTAGCAG19100.021.6200858
CTGACTC20300.020.861382
CACGTCT14000.020.24723414
AGCACAC14750.018.98045710
CGCGGGG2405.020411E-1018.96361
TCCAGTC16300.017.82168225
CCAGTCA16150.017.55396526
GCTGACT25250.017.3315491
CAGTCAC16900.017.18931627
TAGCAGA24850.017.180759
CTAATCG17150.017.14264733
Produced by FastQC (version 0.11.5)