FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_080.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_080.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5900173
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT128170.21723091848323772No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT108650.18414714280411776No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA106540.18057097647814735No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG103660.1756897636730313No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA99250.16821540656519732No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG88300.14965662871241234No Hit
GCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGC79520.1347757091190377No Hit
GGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATG77910.13204697557173323No Hit
CCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCAC77510.13136902934880046No Hit
GGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGG75790.12845386059018948No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGC72550.12296249618443392No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC72540.1229455475288606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68170.1155389850433199No Hit
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATC67750.11482714150924049No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT67680.11470850092022726No Hit
GCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTGA66880.11335260847436168No Hit
CCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTC65840.11158994829473645No Hit
CGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCG63240.10718329784567333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACTC31700.028.595032
CTAGCAG31100.028.2442658
GCTGACT34150.026.6525921
TATGCCG9200.023.20516846
CGTATGC8800.023.06683544
TAGCAGA39100.023.0024099
GTATGCC9500.022.84077345
ACTCTAG44350.022.6470995
GACTCTA43900.021.5240274
GCACACG10100.021.4826211
CTCTAGC44450.020.9427746
ACGTCTG10950.019.8150215
CGGGCGC10500.019.66435215
CGGCAAT14650.018.6386221
TAGCCCG11500.018.56507934
TCGTATG11300.018.2732743
GGCGGGC11400.018.11190413
CACACGT11600.018.10131812
CTATCGA48400.018.0040817
GATCTAT50400.017.42850314
Produced by FastQC (version 0.11.5)