FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_079.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_079.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.7113821138211381No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG50.25406504065040647No Hit
CTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGGACGTAGC40.20325203252032523No Hit
GGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGGACG40.20325203252032523No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG30.1524390243902439No Hit
GCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTGA30.1524390243902439No Hit
CTGGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAAGGCCTACGTGA30.1524390243902439No Hit
CGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGG30.1524390243902439No Hit
GTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGT30.1524390243902439No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC30.1524390243902439No Hit
GAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGGTGGGCCAGGGCA30.1524390243902439No Hit
GGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCG30.1524390243902439No Hit
TGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCC30.1524390243902439No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC20.10162601626016261No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG20.10162601626016261No Hit
CCGTCCTCGGGGTACATCCGCTCGGTGGAGGCCTCCCAGCCCATGGTCTT20.10162601626016261No Hit
GCAAAAGGCAGCACGGGTGTGGCTCGGGACATTTGAGACTGCTGAAGCTG20.10162601626016261No Hit
CGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACG20.10162601626016261No Hit
TGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAG20.10162601626016261No Hit
ATCACCTGACAATTACATAGCACAAAAAGTTATAAAACAGAAGATTATTA20.10162601626016261No Hit
GCAACGGCAGCCTGGCCTGCCTGGCCGCCATGGCCTGGCCCGAGGTCGAG20.10162601626016261No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA20.10162601626016261No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT20.10162601626016261No Hit
CCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAG20.10162601626016261No Hit
CTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGG20.10162601626016261No Hit
GTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAG20.10162601626016261No Hit
TGACGCTTTTTCCAAATAGCTGCAAACGCTGACGCGAGAACTGCAAAGGC20.10162601626016261No Hit
GGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGG20.10162601626016261No Hit
TTTGGCGTTCAAGTTCCTGGTAGCTTCTTCCTCGCTCTGTCCTCCGGATA20.10162601626016261No Hit
CCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCAC20.10162601626016261No Hit
GAAACTTCTCCGACCGTCTTACGGTTCCTTTTGATTGGGAAGAGACTCCA20.10162601626016261No Hit
CTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAGCAGGA20.10162601626016261No Hit
TATCAGGACGATGCTTTCGTTTTCTTTCAATGAAAACATAAATAGTAGTT20.10162601626016261No Hit
TGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTAC20.10162601626016261No Hit
GCACGCCGTAGGTCAGGGTGGTCACGAGGGTGGGCCAGGGCACGGGCAGC20.10162601626016261No Hit
GATGCCCTTCAGCTCGATGCGGTTCACCAGGGTGTCGCCCTCGAACTTCA20.10162601626016261No Hit
CACCAATCTAGACCTCTTAGGAAAAAAACCTAAAAACCTAATCCCCAAAC20.10162601626016261No Hit
ACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGC20.10162601626016261No Hit
ATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGC20.10162601626016261No Hit
CGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACA20.10162601626016261No Hit
CGGCGCCCTGAAGGGCGAGATCAAGATGAGGCTGAAGCTGAAGGACGGCG20.10162601626016261No Hit
ATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACA20.10162601626016261No Hit
CTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGA20.10162601626016261No Hit
GTGACTAGAGACAATCCATCTCCAAGAGCTTACTTCAAATGTGCTTGTGC20.10162601626016261No Hit
CCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAA20.10162601626016261No Hit
GGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCAC20.10162601626016261No Hit
CCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCT20.10162601626016261No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA20.10162601626016261No Hit
CTTCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGA20.10162601626016261No Hit
GGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATG20.10162601626016261No Hit
TTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACC20.10162601626016261No Hit
CGTAATTGTTATTTGATTGAGAAGAAACCCTAGACACGTTATTATTATTA20.10162601626016261No Hit
TCAGATTTGTTGTATATACATACACCATCATTGAGAGATTCCATCCCGGA20.10162601626016261No Hit
GGGGTGTTCTGCTGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGAT20.10162601626016261No Hit
TCTGCTGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGG20.10162601626016261No Hit
AGGGTGGTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCA20.10162601626016261No Hit
GCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTC20.10162601626016261No Hit
GCCGTTTACGTCGCCGTCCAGCTCGACCAGGATGGGCACCACCCCGGTGA20.10162601626016261No Hit
CCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACG20.10162601626016261No Hit
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATC20.10162601626016261No Hit
GCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAG20.10162601626016261No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA20.10162601626016261No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGT20.10162601626016261No Hit
GCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGA20.10162601626016261No Hit
GTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGA20.10162601626016261No Hit
CGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGG20.10162601626016261No Hit
AAGAGGATGGATGTAATGTCAAAGGGTACTTCGTATGGTCGCTATTGGAC20.10162601626016261No Hit
GCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCC20.10162601626016261No Hit
CGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGTCGTAGTGGCCG20.10162601626016261No Hit
CACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAA20.10162601626016261No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC20.10162601626016261No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10162601626016261No Hit
CGATGCGGTTCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTC20.10162601626016261No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC20.10162601626016261No Hit
TGAAGACAATACAAGAAGAGTTGTTGGAACTTTCGGTTACATGCCTCCAG20.10162601626016261No Hit
ACCGCGGTAACGAATGAACGGGTTAAAGGCTCCTTAAAGGTCACAAGAGC20.10162601626016261No Hit
GGACGATCACGATAAGGATCCGATGGTGAGCAAGGGCGAGGAGCTGTTCA20.10162601626016261No Hit
GTCTTCTTCTGCATTACGGGGCCGTCGGAGGGGAAGTTGGTGCCGCGCAG20.10162601626016261No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT20.10162601626016261No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA20.10162601626016261No Hit
CGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCA20.10162601626016261No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC20.10162601626016261TruSeq Adapter, Index 11 (100% over 50bp)
TATCAAGAAGATTGGTAATGCTTTTGACAATATTATCGATGCTAAAAGGA20.10162601626016261No Hit
GCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGGTGGGCC20.10162601626016261No Hit
GCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATTACGGGGCCG20.10162601626016261No Hit
TTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT20.10162601626016261No Hit
AGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTAC20.10162601626016261No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCAA150.001905615270.040
Produced by FastQC (version 0.11.5)