FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_078.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_078.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7311054
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90590.12390826274843544No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA87560.11976385347447852No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT86040.11768480987830209No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT85630.11712401522407029No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG78140.10687925434554307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC13750.026.9802844
TATGCCG14250.026.27902246
ACGTCTG15150.025.4091815
GTATGCC14750.025.3882145
GCACACG15650.024.1501611
CTAGCAG32400.022.2501458
CTGACTC34600.021.2417092
CACACGT18100.020.88121812
TCGTATG18850.020.05149743
CTCGTAT18800.019.73291842
GCTGACT38150.019.7290461
CACGTCT19650.019.23409814
AGCACAC20000.019.07247710
GTCACCC21050.017.9570829
ACTCTAG49800.017.0761725
TAGCAGA43650.016.9164359
CACCCAA24050.016.29945231
AGTCACC23800.016.17632328
CAGTCAC23800.016.17610227
GAGCACA25050.015.9260389
Produced by FastQC (version 0.11.5)