FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_077.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_077.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6579962
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA107340.16313164118576978No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT104620.15899787871115365No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG95870.14569992957406136No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT91800.13951448351829387No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80770.12275146877747928No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA69410.10548693138349433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11100.032.78993646
GTATGCC12300.029.5911445
CGTATGC12600.029.44232444
GCACACG13300.027.8916711
CTAGCAG27600.027.3882928
CTGACTC29450.025.7874182
TCGTATG14500.025.34319543
ACGTCTG14650.025.08218415
CACACGT15850.023.62480212
CTCGTAT15550.022.05696142
AGCACAC17300.021.64501810
GCTGACT35500.021.4968011
CACGTCT17950.020.86089914
TAGCAGA37150.020.7245049
ACTCTAG48250.019.1481535
GCCGTCT19550.018.61730449
TATCTCG18500.018.53976839
CATTTTA19950.018.2454134
GACTCTA47550.018.0313914
AGTCACC21050.017.95663628
Produced by FastQC (version 0.11.5)