FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_076.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_076.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9849997
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT210890.21410158805124507No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG203230.20632493593652873No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA201310.2043756967641716No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT165190.1677056348342035No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA163920.16641629433998814No Hit
GGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATG135230.1372893819155478No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG134890.1369442041454429No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG129010.13097465918009923No Hit
GCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGC127480.12942135921462716No Hit
CCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCAC124860.1267614599273482No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC123870.12575638347910156No Hit
GGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGG116690.11846704115747446No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT112230.1139391209966866No Hit
GCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTGA112150.11385790269783838No Hit
CGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCG112040.1137462275369221No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGC109470.11113708968642326No Hit
GGGGTGTTCTGCTGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGAT103230.10480206237626266No Hit
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATC101550.10309647810045018No Hit
CCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTC101350.10289343235332965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAG47950.026.3470048
GCTGACT49000.026.0772381
CTGACTC50700.025.471032
ACTCTAG59450.022.604355
GACTCTA60450.022.23034
CGTATGC17350.020.77618644
TATGCCG17250.020.69374746
ACGTCTG20800.020.52612315
TAGCAGA63350.020.4946239
GCACACG21200.020.13893911
GTATGCC17900.020.13781245
CTCTAGC66800.019.540926
CACACGT22650.019.15860612
TCTATCG67850.018.25844816
CTATCGA68700.017.93066217
TCGTATG18600.017.49847843
GATCTAT73100.017.23437514
CACGTCT25000.017.21771614
AGCACAC25400.016.8088810
ATCTATC74950.016.71559515
Produced by FastQC (version 0.11.5)