FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_075.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_075.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8196346
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT160810.19619718347663706No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA154900.18898665332088227No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG145250.17721311423407454No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT135310.16508575894673064No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA120620.14716313830577676No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107490.13114380481253476No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG102750.12536074001756392No Hit
GGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATG101710.12409188191908932No Hit
GCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGC98350.11999249421632517No Hit
CCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCAC95570.11660073891463342No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC95310.11628352439001478No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGC90480.11039065456729133No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT89510.10920720037929096No Hit
GGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGG87790.10710870429335219No Hit
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATC82940.10119143335335037No Hit
CCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTC82000.10004458084126755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16200.033.70025346
CGTATGC17450.031.8882444
GTATGCC17150.031.42554745
CCAGGCG17500.030.39892833
GCACACG20600.026.50133111
ACGTCTG21750.025.74370415
ACCAGGC20950.025.7273332
CTAGCAG37600.025.5950038
CAGGCGA22350.024.42866934
CTCGTAT20400.024.18958742
CTGACTC39150.024.0472432
TCGTATG22750.023.99783543
CACACGT22750.023.84298512
GCTGACT41400.023.5077551
AGCACAC24600.023.04571710
CACGTCT25550.021.91489614
GCGATCT23050.021.56053537
ACACGTC25600.021.46199213
ACTCTAG49150.021.0045855
TAGCAGA47300.020.4201189
Produced by FastQC (version 0.11.5)