FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_074.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_074.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7639152
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA117240.15347253202973316No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG112000.14661313192877953No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT109570.14343215058425335No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99990.1308914916210595No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT90010.11782721432954861No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA86090.11269575471204135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14550.032.4711346
GTATGCC15800.029.68109945
CGTATGC16050.029.4368344
GCACACG17300.028.32007411
ACGTCTG18650.026.08227715
CACACGT20650.023.89508812
TCGTATG19850.023.80156943
CTCGTAT20150.023.62119542
AGCACAC21900.023.0107510
CTAGCAG28700.022.5580848
CACGTCT22550.021.72656214
CTGACTC32400.020.6316932
CAGTCAC25200.018.88832327
AGTCACC25750.018.62080228
ACACGTC26700.018.61172713
GCTGACT35150.018.4256881
TCCAGTC28100.017.93517125
CCACTCA26700.017.56547733
GAGCACA29900.017.5562789
CGCGGGG4450.017.3077241
Produced by FastQC (version 0.11.5)