FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_073.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_073.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10628884
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT244260.2298077578041119No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA243960.2295255080401668No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG231220.2175393013979643No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT181840.17108099025259849No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA167740.1578152513471781No Hit
GGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATG161540.1519820895589791No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG149400.14056038244466681No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC148090.13932789180877314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138060.1298913413675415No Hit
GCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGC137640.12949619169801835No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT131900.12409581288120182No Hit
CCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCAC126380.11890241722461173No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGC125230.11782045979615546No Hit
GGGGTGTTCTGCTGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGAT125130.1177263765415071No Hit
GGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGG121810.11460281248718115No Hit
CGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCG113280.10657751086567509No Hit
GCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTC111340.10475229572549666No Hit
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATC111060.10448886261248122No Hit
CCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTC110620.1040748962920284No Hit
GCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTGA110350.1038208715044778No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC109210.10274832240148636TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22950.034.9204546
CGTATGC24350.033.34387244
GTATGCC25600.031.71590245
GCTGACT48900.029.852091
GCACACG28950.028.64919911
ACGTCTG30300.027.95010215
CTCGTAT29500.027.64210542
CACACGT31050.027.2749812
TCGTATG30750.026.97280543
CTAGCAG54750.026.7180548
ACTCTAG58200.025.675545
CTGACTC56950.025.318662
GACTCTA61600.024.087844
AGCACAC35750.023.6892910
TCTCGTA34900.023.46538441
ACACGTC36600.022.8521713
CACGTCT36950.022.54099814
ACGATAT36600.022.18494835
GCCGTCT36700.022.12335449
CGGGGGT11100.022.0761151
Produced by FastQC (version 0.11.5)