FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3832770
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG149670.3905008649097128No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC110440.2881466928618206No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG78300.204290891444047No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT78300.204290891444047No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC63620.1659896106471299No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC63010.1643980724123806No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG62660.16348489473670477No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG59050.15406611928187708No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA58690.15312685081546767No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG54920.14329062270890244No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC50380.13144540371585042No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC49190.12834059961855265No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48200.12575761133592675No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA46390.1210351782131461No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA43340.11307748703939971No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT43150.1125817620154614No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG40820.10650260777453384No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG40120.1046762524231822No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG39120.10206717334982271No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC38940.10159753911661801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7900.035.88217544
GTATGCC8150.034.35254345
GCACACG9550.031.88052211
TATGCCG8800.031.81514446
ACGTCTG10200.028.81964515
ATGCCGT9850.028.4236847
GCTGACT18700.028.2722381
CTCGTAT10200.027.10525142
TCGTATG10650.026.61682743
AGCACAC11900.026.46702210
CACACGT11500.025.86608112
CACGTCT11750.025.01790614
CTGACTC21550.025.0095352
ACACGTC11900.024.70255513
CTAGCAG21200.024.5957438
CCGGGGT3200.024.071191
ACTCTAG29100.023.8112265
GCCGTCT12100.023.7164349
GTGCGCA49900.022.10649568
TGCGCAT51050.021.60850569
Produced by FastQC (version 0.11.5)