FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_070.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_070.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7089031
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG229430.32364084738802806No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC216580.30551425152464423No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG165310.23319124997478502No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT120070.16937434749544755No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC113610.16026167751276585No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG112280.15838553957515492No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC110710.15617085043075704No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG105120.14828542857267799No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC97970.13819942387048387No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG96680.13637971113400407No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA96410.13599884102636878No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA95610.13487033700374565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95600.13485623070346286No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA85160.12012925320823115No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC84040.11854934757655877No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG78640.11093194542385271No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG73570.10378005118047869No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT71510.10087415332222416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC12200.030.1195646
TATGCCG12150.029.66723348
GTATGCC13100.028.05027847
CTAGCAG31350.026.5668588
CTGACTC35150.025.090522
ATGCCGT14550.025.01401349
ACGTCTG15750.024.66267415
GCTGACT35600.024.1912021
GCACACG17450.023.0623311
CTCGTAT16150.022.3194744
TCGTATG17250.021.50482745
GTGCGCA76500.021.29043868
TATCTCG17450.021.25850141
ACTCTAG45050.020.8957655
TGCGCAT78900.020.64354769
TAGCAGA42100.020.2818959
CACACGT19250.020.1785512
GGTGCGC84400.019.62711767
AGGTGCG83800.019.55661666
GACTCTA45800.019.4837444
Produced by FastQC (version 0.11.5)