FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_069.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_069.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7691629
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG277640.36096384784029495No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC236590.30759413903088667No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG175310.22792310965596496No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT144260.18755454793776455No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC130460.1696129649518977No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC130000.16901491218570214No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG121300.1577039142163513No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG120820.15707985915597333No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA111690.14520981186170054No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG109530.14240156408999965No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC105310.13691508001750996No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA102540.13331376227324537No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97810.12716421969910405No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA92430.12016960256403422No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC90100.11714033529178279No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG85470.11112080418855357No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG79800.1037491537878387No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG79440.10328111249255521No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC78530.1020980081072553No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT77660.10096690831032022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACTC40650.028.6700172
GCTGACT41550.028.4775681
GTATGCC12050.028.46187845
CTAGCAG41200.028.2852658
CGTATGC12300.028.16790844
TATGCCG12550.027.60643646
CGCGGGG4400.024.6641541
GCACACG15750.023.99697111
ACTCTAG54650.023.6934135
ACGTCTG16350.023.54442815
TAGCAGA49950.023.1202039
GACTCTA53800.022.6366944
GTGCGCA88100.022.54042668
TGCGCAT90850.022.05103169
CTCTAGC53250.022.0161446
CACACGT17250.021.70740512
ATGCCGT17200.021.16039547
AGGTGCG94150.021.08829166
GGTGCGC96500.020.83024267
CACGTCT18650.020.82847214
Produced by FastQC (version 0.11.5)