FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_068.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_068.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8029030
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG256370.31930382624052966No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC197940.24653040280083643No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG150680.1876689961302922No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT137320.17102937714767535No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC118100.14709124265322213No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC115740.14415190876108322No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG111550.13893334562207388No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109600.136504658719671No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG106250.1323322991693891No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG105110.13091245144183047No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA99030.12333993022818447No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC92490.11519448800166396No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC86490.10772160522503965No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA85450.10642630554375809No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA81720.1017806634176233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC13600.030.36471247
TATGCCG13450.029.92313248
CGTATGC14000.028.99701746
CTGACTC37550.026.7484132
CTAGCAG38150.026.6016068
ACGTCTG17250.025.76450315
TCGTATG17800.023.98644445
GCACACG18800.023.82629811
GCTGACT43600.023.2045271
ATGCCGT17900.022.48414249
GTGCGCA83600.022.24228968
CACACGT20150.022.05632212
TGCGCAT85200.021.7841769
TAGCAGA47050.021.6440149
CTCGTAT19400.021.10594644
TATCTCG19500.020.63954541
CACACTG20650.020.33904531
CACGTCT22200.020.3348614
AGGTGCG91050.020.30532866
GGTGCGC94600.019.91434967
Produced by FastQC (version 0.11.5)