FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_067.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_067.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6899066
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG129960.18837332473700064No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC116350.16864601672168378No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG86570.12548075348170318No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86210.12495894371788877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12050.029.33352946
CGTATGC13100.027.78402744
GTATGCC13050.027.35412445
GCACACG14550.025.49549311
GGTAGCA15800.022.5944334
ATGCCGT16050.022.24104347
ACGTCTG16550.021.99155415
TCGTATG16850.021.39278643
CTCGTAT16650.021.22952742
CACACGT17400.021.11838212
GTGCGCA44550.020.43669568
AGTCACG18150.020.05435828
CTAGCAG24600.019.9165218
TGCGCAT46950.019.54188569
CACGTCT20150.018.75725414
AGCACAC19650.018.70024510
CTGACTC27150.018.3041062
GTAGCAT19950.018.0695135
GGTGCGC54200.017.63668367
AGGTGCG52450.017.35626266
Produced by FastQC (version 0.11.5)