FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_066.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_066.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7658897
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG304970.3981904966211192No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC224140.2926531065765736No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG174590.22795710661731053No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT146490.19126775043455996No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC120150.15687637527962578No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC116430.15201927901628656No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG115250.15047858719081875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111400.14545175369247035No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG110520.14430276317856214No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA109090.1424356535934613No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG108900.14218757609614022No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA95280.1244043365513337No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC92410.12065706067074672No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC89410.11674004755515056No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA86710.11321473575111403No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG79970.10441451295140802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT37300.025.4355681
CTAGCAG39300.024.0423228
GTGCGCA100150.023.1844568
CTGACTC41800.022.941662
TGCGCAT102800.022.65611569
GTATGCC11750.022.63590847
ACTCTAG45600.022.1020645
GACTCTA46600.021.9281564
CGTATGC12150.021.8905546
AGGTGCG107950.021.50614766
GGTGCGC108450.021.47325767
TATGCCG12600.021.38663148
GCACACG14300.020.31174511
AAGGTGC122850.018.9535265
TAGCAGA51300.018.6230359
CTCTAGC52900.018.2582266
ATGCCGT15050.018.13738449
CTCGTAT16150.017.3356344
AGTCACG15800.017.27823428
ACGTCTG16250.017.22827715
Produced by FastQC (version 0.11.5)