FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7574499
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG226970.2996501814839503No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC214300.282923002564262No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG172040.2271305336498163No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC117000.15446566168930775No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC113190.14943562603942517No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT111700.1474684992367152No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG106630.14077498723017853No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG105020.13864943410778718No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99970.1319823264878641No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG97040.12811408384897802No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC96210.1270183018045154No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA93960.12404780831049024No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA88030.11621890767957062No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC86760.11454222912960976No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA82230.10856163556163913No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG81470.10755826887032396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC11050.031.03715146
CGTACGT10850.030.6446734
GTACGTA11350.028.67700835
TATGCCG11950.028.40677348
CCGTACG12300.027.03190833
CTAGCAG32100.026.0553328
GTATGCC13150.025.8145247
CGCGGGG3750.024.2710861
TACGTAA13850.024.00604236
GCTGACT35950.023.6621651
ATGCCGT14850.023.0946849
TCGTATG15300.022.87318645
CTGACTC36950.022.8264242
GCACACG16350.021.61759211
GTGCGCA74400.020.94832868
TGCGCAT77250.020.2674969
CTCGTAT17100.020.26069344
TAGCAGA43850.019.5524069
ACGTCTG17800.019.4634115
AGGTGCG80650.019.36426266
Produced by FastQC (version 0.11.5)