FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_064.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_064.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493335
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG91290.36613611889296865No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC75410.30244632189416987No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG55180.22131001249330717No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT45690.1832485406092643No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC39880.1599464171481169No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG38500.1544116614895311No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA37500.1504009689833095No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC37260.14943840278181633No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG35450.14217904934555525No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34270.13744643218821379No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG34220.1372458975629027No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC30080.12064163058714533No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA29940.1200801336362743No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC28970.11618976190523936No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG28780.11542773032905727No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG28430.11402398795187971No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA26800.10748655916673852No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC25810.10351597358557915No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT24950.10006677803022859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC5100.035.68282747
CGTATGC5300.035.6576546
TATGCCG5450.033.39127348
CGCGGGG1900.029.4808921
CTCGTAT6500.029.074744
GCACACG6950.028.70053911
GTCACGT6950.027.69850229
ACGTCTG7150.027.4082915
ATGCCGT6650.027.36522949
AGTCACG7100.027.11332128
CTAGCAG12550.026.7687138
TCGTATG7150.025.94207245
CTGACTC13400.025.8557112
GCCGTCT7100.025.63081651
CACGTCT8050.025.2134414
CACACGT8100.024.6257712
GCTGACT14250.024.3217331
AGCACAC8850.023.72509410
CGTTTCG8250.023.33388733
CACGTTT8350.023.0544431
Produced by FastQC (version 0.11.5)