FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_063.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_063.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4963088
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG159580.3215336903153843No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC103040.2076126798477077No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT83350.1679397987704429No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG77790.15673709593704566No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75900.15292898292353468No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC66210.1334048479495024No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC65470.1319138407378632No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG65250.1314705683235921No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG62560.12605055562182255No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA61400.12371330107384756No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG59570.12002608053695601No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC50160.10106611045381424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8500.033.34920548
CGTATGC9250.031.02386546
CTAGCAG21750.029.9261748
GTATGCC9800.029.28243647
AGTCACG10850.028.0633528
ATGCCGT10550.027.19992349
CTGACTC23950.026.1559982
ACGTCTG11600.025.94432615
GTCACGT11700.025.42655429
GGCCTTA11500.024.65065636
CTCGTAT11900.024.11567544
TCACGTG12450.023.89483530
TCGTATG12100.023.7168345
GCCTTAT11850.023.62723537
GCTGACT27200.023.1640721
GCACACG13200.023.06443611
TAGCAGA29050.022.2855389
CGCGGGG2501.8189894E-1221.0020921
CACACGT14500.020.9965912
CACGTGG14350.020.9749531
Produced by FastQC (version 0.11.5)