FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_062.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_062.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8951552
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT148550.16594887679812395TruSeq Adapter, Index 19 (97% over 40bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG135440.15130337175050762No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114920.12837997254554295No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC105040.11734278033574515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC27000.042.2557146
TATGCCG27050.042.17760548
GTATGCC28750.040.29226347
ACGTCTG31650.037.26218815
TCGTATG31050.036.74429745
GCACACG32200.036.19119611
CTCGTAT30800.035.79244644
ATGCCGT31950.035.7090549
GTCACGT31750.035.4954629
AGTCACG33300.033.94799428
CACACGT35650.032.78680412
CACGTCT36500.032.40678814
TCACGTG35650.031.61236630
GCCGTCT36150.031.3666651
CACGTGA37150.030.52438231
AGCACAC39200.029.90703210
CAGTCAC39400.029.31389627
TCCAGTC42550.027.5538125
CCAGTCA43850.026.41857326
CTGAACT45950.026.19946119
Produced by FastQC (version 0.11.5)